Coding
Part:BBa_K212005
Designed by: Elias Scheer Group: iGEM09_Brown (2009-10-20)
TrgEnvZ
transduces RBP signal to OmpC promoter
Usage and Biology
TrgEnvZ is an archetypal two-component regulatory system that has been studied by Dr. Masayori Inouye since the early 1990s. It constitutes a chimeric protein composed of the extracellular and transmembrane domains of chemoreceptor Trg and the kinase domain of osmosensor EnvZ. Trg is activated by periplasmic binding proteins, which in turn transduces the message to EnvZ to phosphorylate OmpR, which binds to the OmpC promoter, driving transcription of whatever gene is under it.
Adapted from Ref. 1 Figure 1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 348
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |